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KMID : 1094720130180051038
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Biotechnology and Bioprocess Engineering
2013 Volume.18 No. 5 p.1038 ~ p.1047
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Development of candidate biomarkers for pancreatic ductal adenocarcinoma using multiple reaction monitoring
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Yu Ji-Young
Kim Kyung-Gon
Kang Mee-Joo
Kim Hyun-Soo
Kim Sun-Whe
Jang Jin-Young
Kim Young-Soo
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Abstract
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Pancreatic ductal adenocarcinoma (PDAC) is the fourth most frequent cause of cancer mortality in the United States. Because CA 19-9 increases not only in PDAC, but also in benign conditions, there is urgent need for an additional PDAC biomarker. Isotope tags for relative and absolute quantification (iTRAQ) were performed using 6 pairs of PDAC and normal tissues from the same patients, to obtain preliminary PDAC-specific proteins; and verification was performed by multiple reactions monitoring (MRM), using 30 PDAC and 20 normal serum, targeting high-abundant serum proteins without any pre-preparation. As a result, 17 candidate proteins from tissue iTRAQ were verified as potential markers (AUC values > 0.7). Multivariate analysis (MA) demonstrated that a 6-marker panel, consisting of alpha-1 antitrypsin, haptoglobin beta chain, hemopexin, transferrin, zinc alpha-2 glycoprotein, and apolipoprotein A4 from the MRM result, had comparable discriminatory power versus CA 19-9. Our study demonstrated that a combination of iTRAQ on PDAC tissue and verification MRM-MA on individual serum was an efficient method for the development of PDAC multimarkers.
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KEYWORD
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pancreatic ductal adenocarcinoma, LC-MS/MS, multiple reaction monitoring, cancer biomarker, proteomics
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